RNA Melting Analysis
Two-state model thermodynamics
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Drop your melting data CSV
Column 0 = temperature (°C) · Columns 1+ = signal
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Melting curve · raw + two-state fit + baselines
Van't Hoff · ln(Ka) vs 1000/T
dA/dT derivative · Tm estimate
Fit results — two-state model
Results per column
| Column | Tₘ raw (°C) | Tₘ vH (°C) | ΔH vH (kcal/mol) | ΔS vH (cal/mol·K) | ΔG₃₇ vH (kcal/mol) | Tₘ fit (°C) | ΔH fit (kcal/mol) | ΔS fit (cal/mol·K) | ΔG₃₇ fit (kcal/mol) | Tₘ multi (°C) | ΔH multi (kcal/mol) | ΔS multi (cal/mol·K) | ΔG₃₇ multi (kcal/mol) |
|---|
Van't Hoff — concentration series
| Column | CT (µM) | ln(CT/f) | Tₘ raw (°C) | 1/Tₘ raw (K⁻¹) | Tₘ vH (°C) | 1/Tₘ vH (K⁻¹) | Tₘ fit (°C) | 1/Tₘ fit (K⁻¹) |
|---|
Linear regression: 1/Tm = (R/ΔH)·ln(CT/f) + ΔS/ΔH
| Method | n | slope (K⁻¹) | intercept (K⁻¹) | ΔH° (kcal/mol) | ΔS° (cal/mol·K) | ΔG°₃₇ (kcal/mol) | R² |
|---|